The 2-Minute Rule for thr77 login
The 2-Minute Rule for thr77 login
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in many tumors, which leads to chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was found to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a crucial function in marketing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two additional splice variants ended up described but comprehensive nucleotide sequence information just isn't nevertheless offered.
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Blocking peptides are peptides that bind specially for the target antibody and block antibody binding. These peptide ordinarily has the epitope acknowledged from the antibody.
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This guide outlines even further possible optimization of cellular and immunoassay parameters to make certain the very best outcomes are...
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This guidebook outlines more achievable optimization of cellular and immunoassay parameters to guarantee the best possible outcomes are...
Antibodies certain to the blocking peptide not bind into the epitope within the focus on protein. By comparing the staining from your blocked antibody versus the antibody by itself, you can see which staining is distinct.
Focus on Details Cyclin E belongs to your extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic event. Cyclin E forms a fancy with and functions as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells progress through S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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